The Escherichia coli glycophage display system.
نویسندگان
چکیده
We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylation.
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ورودعنوان ژورنال:
- Glycobiology
دوره 20 11 شماره
صفحات -
تاریخ انتشار 2010